Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PGR

Cell type

Cell type Class
Uterus
Cell type
Uterin leiomyoma
NA
NA

Attributes by original data submitter

Sample

source_name
Primary uterine leiomyoma cells
tissue preparation
Fresh frozen tissue
patient id
Patient 3
cell type
uterine leiomyoma
chip antibody
anti-PGR Santa Cruz (sc-7208X)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
0.2-0.5g of fresh frozen LM tissues (patient number = 5) were used for ChIP-Seq using the SimpleChIP Kit (Cell Signaling Technology, #9005). PGR antibody was purchased from Santa Cruz (#sc-7208X). Next-generation sequencing libraries were prepared using the KAPA Hyper Prep Kit (KAPA Biosystems, KK8502) and KAPA Single-Indexed Adapter Kit (KAPA Biosystems, KK8710).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
50223765
Reads aligned (%)
98.0
Duplicates removed (%)
6.0
Number of peaks
6422 (qval < 1E-05)

hg19

Number of total reads
50223765
Reads aligned (%)
97.2
Duplicates removed (%)
7.4
Number of peaks
6298 (qval < 1E-05)

Base call quality data from DBCLS SRA