Antigen

Antigen Class

TFs and others

Antigen

PGR

Cell type

Cell type Class

Uterus

Cell type

Uterin leiomyoma

NA

NA

Sample

source_name

Primary uterine leiomyoma cells

tissue preparation

Fresh frozen tissue

patient id

Patient 3

cell type

uterine leiomyoma

chip antibody

anti-PGR Santa Cruz (sc-7208X)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

0.2-0.5g of fresh frozen LM tissues (patient number = 5) were used for ChIP-Seq using the SimpleChIP Kit (Cell Signaling Technology, #9005). PGR antibody was purchased from Santa Cruz (#sc-7208X). Next-generation sequencing libraries were prepared using the KAPA Hyper Prep Kit (KAPA Biosystems, KK8502) and KAPA Single-Indexed Adapter Kit (KAPA Biosystems, KK8710).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

50223765

Reads aligned (%)

98.0

Duplicates removed (%)

6.0

Number of peaks

6422 (qval < 1E-05)

hg19

Number of total reads

50223765

Reads aligned (%)

97.2

Duplicates removed (%)

7.4

Number of peaks

6298 (qval < 1E-05)